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Pokérus is a virus in the game series that any Pokémon can catch if it has not already been infected once before.
Pokérus is caught when a Pokemon battles a wild pokemon. The effects of this virus are very beneficial to the Pokémon that receives it. Catching the virus however, is a very rare event, as the odds of meeting a wild Pokémon with it is 1 to 21,845 or roughly 0.0000457%. Similar to the Macho Brace, Pokérus will double the EV (effort values) stats of a Pokémon. A player can combine a Macho Brace and Pokérus to get a substantial amount of EV's.
When caught, the Nurse in a Pokémon Center will inform you if you have received it. Also, in HG and SS, Professor Elm. will call you and say something around "You got the Pokerus. Don't worry, it will wear off."
The Pokérus is spread through training and battling Pokémon who do not have Pokérus with a Pokémon who does have it. The virus does not last forever, after two days or so it will wear off of the Pokémon and it will not be able to be received again, however, if placed in the PC box, Pokérus will last until you take it out. There is 25% chance for a Pokémon to catch a Pokérus.
After the timer has run out for Pokérus you still get the benefits. However you don't get to spread it around. It is advisable to try and keep Pokérus by putting them into PC BOX before midnight (12:00) overwise you will lose Pokérus.
Pokémon with the pokerus will lose pokerus eventually, and a small dot will appear in your Pokémon's status screen. And the small dot on your Pokemon's status means it's protected to Pokerus.
Have the Pokemon with Pokerus in the middle (third slot). Keep on battling until your entire team gets Pokerus. If the last Pokemon in your party did not get Pokerus simply switch it so that it is closer to the rest of your Pokemon. It is always a good idea to have at least three Pokemon with Pokerus in your PC Box.